By Matthew Delooze
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Additional info for The Answer, My Friend, Is Blowing The Wind
2008). Furthermore, overexpression of ADAM10 in early lymphocyte progenitors under the H-2 Kb promoter and the IgH enhancer region, exhibited a smaller thymus, reduced DN and DP thymocyte populations and other B and myeloid specific defects (Gibb et al. 2011). In contrast, proteolytic processing of Notch ligands is not critical during T cell development. Using an uncleavable Dll1 overexpressed in OP9 cells, T cell development was analyzed and found to progress normally (Gravano and Manilay 2010).
Dev Dyn 235:886–894 Ciofani M, Zuniga-Pflucker JC (2005) Notch promotes survival of pre-T cells at the betaselection checkpoint by regulating cellular metabolism. Nat Immunol 6:881–888 Ciofani M, Zuniga-Pflucker JC (2007) The thymus as an inductive site for T lymphopoiesis. Annu Rev Cell Dev Biol 23:463–493 Notch Receptor-Ligand Interactions During T Cell Development 39 Ciofani M, Schmitt TM, Ciofani A, Michie AM, Cuburu N, Aublin A, Maryanski JL, Zuniga-Pflucker JC (2004) Obligatory role for cooperative signaling by pre-TCR and Notch during thymocyte differentiation.
This force would presumably have to be created in the signal-sending cell and involve other proteins, such as Epsin, Dynamin, and Clathrin, which have all been implicated in generating a mechanical force (Liu et al. 2010; McMahon and Gallop 2005) and shown to have a role in Notch ligand endocytosis. Notch receptor 30 D. K. Shah and J. C. Zúñiga-Pflücker binding to ligand induces ubiquitinylation of the Notch ligand, and recruitment of Epsin (Overstreet et al. 2004; Tian et al. 2004; Wang and Struhl 2004).
The Answer, My Friend, Is Blowing The Wind by Matthew Delooze