By Abelson J. (ed.), Simon M. (ed.), Sies H. (ed.)
The looks of one other quantity in that first-class sequence, tools in Enzymology, is usually a reason for appreciation when you desire to effectively perform a selected process or arrange an enzyme or metabolic intermediate with no the tiresome prospect of looking through unusual literature and maybe making a choice on an unproven procedure which isn't simply reproduced.
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Additional info for Retinoids: Cell Differentiation and Clinical Applications Part B
Extract the pellet in 2 ml chloroform, centrifuge at room temperature, and transfer the supernatant to the collect tube. 6. 5% KCI to the collected supernatants, vortex, add 2 ml water, centrifuge 5 rain at 900 g at room temperature, and transfer the underlying fluid to a second collect tube. 7. Wash the remaining upper layer with 4 ml chloroform, centrifuge 5 min at 900 g at room temperature, and transfer the underlying fluid to the second collect tube. 8. Evaporate organic solvents from the second collect tube to dryness at 50 ° under a stream of nitrogen.
5 #1 ethanol/ml medium only. 14p. W. Wertz, M. C. Miethke, S. A. Long, J. S. Strauss, and D. T. Downing J. Invest. Dermatol. 84, 253 (1985). 15p. W. Wertz, E. S. Cho, and D. T. Downing, Biochim. Biophys. Acta 753, 350 (1986). 16p. A. Bowser, D. H. Nugteren, R. J. White, U. M. T. HoutsmuUer, and C. Prottey, Biochim. Biophys. Acta 834, 419 0985). 17p. A. Bowser, R. J. White, and D. H. Nugteren, Int. J. Cosmet. Sci. 8, 125 (1986). ~sj. G. Rheinwald, in "Methods in Cell Biology" (C. Harris, B. F. Trump, and G.
Estimation of Relative Cholesterol Sulfate Content Ceils in culture take up radioactive sulfate from the culture medium and incorporate sulfate into a variety of cellular components which include cholesterol sulfate. Isolation and quantitation of cholesterol [35S]sulfate provides a measure of cholesterol sulfate content which can be compared between cells grown under various culture conditions. 1 M KC1/methanol, 1 : 1 (v/v) Silica gel G plates (Analtech, Newark, DE) Procedure Incorporation of[3~S]Sulfate into Cells.
Retinoids: Cell Differentiation and Clinical Applications Part B by Abelson J. (ed.), Simon M. (ed.), Sies H. (ed.)