Gas Chromatography and Lipids: A Practical Guide - download pdf or read online

By William W. Christie

ISBN-10: 095141710X

ISBN-13: 9780951417102

Fuel chromatography used to be first constructed through lipid analysts and lipid analysts were on the leading edge within the improvement of the process.

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The collector electrode is cylindrical and is placed just above the flame, and the ion current is measured by establishing a potential between the collector and the jet tip. In order to prevent the ions from recombining, a potential is selected in the saturation region, where increasing the potential has relatively little effect on the ion current. The signal current is passed to an amplifier, which must have a linear range to match that of the detector itself, and thence is transmitted to a recorder.

5. 4. Schematic diagram of a typical split/splitless injection system for WCOT columns. 4. On-column injection In on-column injection, the sample is injected in a solvent directly onto the column. The sample is concentrated by "cold trapping" or a "solvent effect" at the head of the column, so must be injected at a column temperature near the boiling point of the solvent. The column inlet does not have a septum but rather a "duck-bill" valve, made of a soft elastomer. This consists simply of two plastic surfaces which are pressed together by the pressure of the carrier gas in the injection port.

E. column length, column internal diameter and film thickness. Of these, column length is least important as it can be established that resolution is proportional only to the square root of column length. Thus to improve by a factor of two on the resolution attainable with a 25 m column, it would be necessary to move to one 100 m in length, and this would inevitably mean that the analysis time would be increased by a factor of four. In contrast by reference to the last equation above, it is apparent that changes in the column internal diameter or in the film thickness have a marked effect on the partition ratio.

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Gas Chromatography and Lipids: A Practical Guide by William W. Christie

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