By ur-Rahman, Atta- Caldwell, Gary W. Choudhary, M. Iqbal
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Extra resources for Frontiers in Drug Design and Discovery, Volume (4)
C: The reaction of GSH with nitroalkanes catalyzed by GSTs. Several GSTs catalyze the conjugation of the GSH with alkyl halides and related compounds . For example, conjugation of the drug chlorobucil is an example for this type of reaction. Examples of the GSH conjugations catalyzed by GSTs are presented in Fig. (4). GSH conjugations of alkyl halides can be detoxication or bioactivation reactions. In the later case the substrate is activated leading to reactions with other nucleophiles, particularly DNA and proteins.
A special recognizable feature of the mu class GSTs is the socalled mu loop, which is the result of an insertion in N-terminal domain. Another structural attribute, which is recognizable in both mu and pi classes of GST, is the C-terminal wall . On average, the C-terminal end is 4 to 8 amino acid residues shorter than their alpha counterpart and forms a wall that results in a partially blocked access to the xenobiotic binding site . In addition, the pi and mu classes share a larger H-site that is more accessible to solvent entry than the alpha class GSTs.
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Frontiers in Drug Design and Discovery, Volume (4) by ur-Rahman, Atta- Caldwell, Gary W. Choudhary, M. Iqbal