By Radford D.E., Souza F.J.O., Yetter D.N. (eds.)
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HIGH-PERFORMANCE PRECOATED STATIONARY PHASES would be difficult to achieve by other separation mechanisms. Reversible weak charge-transfer complexes are also thought to be responsible for the separation of polycyclic aromatic compounds by ring number on silica gel layers impregnated with caffeine. 4 Layer Pretreatment Precoated inorganic oxide layers are invariably contaminated, or quickly become so when opened and handled in the laboratory. They contain residual contaminants from the manufacturing process and contaminants acquired by contact with packaging materials and by adsorption from the atmosphere.
A single or double 5-min immersion in a polar solvent is generally superior to ascending development for removing contaminants. For trace analysis, both sequential ascending development followed by immersion may be required for acceptable results. Some manufacturers now offer especially clean precoated layers for trace analysis and mass spectrometric detection. Reproducible retention on inorganic oxide layers requires careful control of the layer activity. This is achieved by the adsorption of reagents, most notably water, through the gas phase .
Native cellulose 46 2. HIGH-PERFORMANCE PRECOATED STATIONARY PHASES consists of 2e20 mm fibers with 400e500 monomer units. Microcrystalline cellulose is prepared by the acid hydrolysis of native cellulose with a narrower monomer distribution (in the range 49e200 monomer units) and a rod-shaped structure. Both forms of cellulose have surface areas of about 2 m2/g. Ion-exchange celluloses are prepared by surface modification to introduce aminoethyl (AE), carboxymethyl (CM), diethylaminoethyl (DEAE), and phosphate (P) groups.
Diagrammatic Morphisms and Applications by Radford D.E., Souza F.J.O., Yetter D.N. (eds.)