Read e-book online A Laboratory Manual of Analytical Methods of Protein PDF

By P. Alexander, H. P. Lundgren

ISBN-10: 0080113982

ISBN-13: 9780080113982

Show description

Read or Download A Laboratory Manual of Analytical Methods of Protein Chemistry. Volume 4 PDF

Best nonfiction_12 books

New PDF release: The Mysterious Island: Dropped From the Clouds (1875)

The Mysterious Island tells the fascinating tale of 5 americans stranded on an uncharted island within the South Pacific. through the American Civil battle, Richmond, Virginia used to be the capital of the accomplice States of the USA. 5 northern prisoners of struggle choose to get away Richmond in a slightly strange method - through hijacking a balloon.

Extra resources for A Laboratory Manual of Analytical Methods of Protein Chemistry. Volume 4

Example text

It is also conventient to keep all such reagents in plastic or stainless steel trays. A similar tray, of about 4 square feet may be used to hold the polarographic and calomel cell assembly. These precautions apply equally well to work with all highly-reactive —SH reagents particularly the volatile ones like iodoacetate and alkyl halides. All of these reagents are toxic on inhalation and have a vesicant action on the skin at high concentrations. None must be pipetted by mouth. Using the above precautions, MeHgl and the other reagents mentioned have been in constant use in the reviewer's laboratory by 6 or 8 workers for nearly 10 years without any reported ill effects.

With most samples reaction will be complete in 1 or 2 hr but with particularly refractory samples, reaction may require as long as 72 hr before it is complete. In the great majority of cases, 24 hr is recommended as a satisfactory period. When insoluble samples are used, the flasks should be examined after the first hour or two to ensure complete wetting and immersion in the mercurial solution. It is sometimes necessary to apply suction to remove adherent air bubbles. After reaction, the solution is decanted non-quantitatively into the polarographic cell* so * More complete details of the operation of the polarographic cell and of precautions to be observed, are to be found in method (b).

None must be pipetted by mouth. Using the above precautions, MeHgl and the other reagents mentioned have been in constant use in the reviewer's laboratory by 6 or 8 workers for nearly 10 years without any reported ill effects. MeHgl (5 x 10~4M)—Solution A: This solution is required for measuring the rate and final extent of reaction of —SH groups with excess MeHgl. Since dénaturants are absent, any changes in biological or physicochemical properties of a protein treated with this solution may be attributed to reaction of the —SH groups alone.

Download PDF sample

A Laboratory Manual of Analytical Methods of Protein Chemistry. Volume 4 by P. Alexander, H. P. Lundgren


by David
4.2

Rated 4.10 of 5 – based on 7 votes